Is the 3´→ 5´ exonuclease activity of Klenow Fragment present when the length of DNA 3'-overhang is over 10 base?

30 December 2015 1 9K Report

can the 3´→ 5´ exonuclease activity of Klenow Fragment still be valid when the length of DNA 3'-overhang is over 10 bases. And what if the 3'-end has been Phosphorylated, does it remain hydrolyzable ?

Syed A Ali

Yes and yes.

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