Wang Xuchu

5 Questions 5 Answers 0 Followers

Questions related from Wang Xuchu

Adaptor ligation and purification during the construction of cell free DNA library. How can I solve It?

Hi, recently I am trying to build up a DNA library derived from circulating DNA. I used two-step strategy: 1) cell free DNA were phosphorylated using T4 Polynucleotide Kinase (first...

09 June 2021 4,223 1 View

Which component should I use to avoid unspecific binding after magnetic bead - antibody conjugation ?

I want to conjugate anti - CD9 antibody onto magnetic bead (diametre: 7 nm ). I used 10% BSA to block unspecific protein binding after conjugation. But the results showed unexpected adhesion...

01 December 2017 9,407 7 View

Isolation exosome from serum by Exosome Isolation Kit CD9, human ( miltenyi)

Hi, I isolated exosomes by Exosome Isolation Kit CD9, human ( miltenyi), and the TEM image after procedures is attached below. Can you tell me whether the exosomes are isolated ? Thank you so much.

22 June 2017 3,285 4 View

Can anyone suggest protocols for Polyethylene Glycol (PEG) or Triethylene Glycol (TEG) linking between two oligonucleotide strand ?

I want link two single DNA strand by PEG or TEG units. Can someone suggest any feasible protocols. Thanks very much.

24 July 2016 2,787 3 View

Is the 3´→ 5´ exonuclease activity of Klenow Fragment present when the length of DNA 3'-overhang is over 10 base?

can the 3´→ 5´ exonuclease activity of Klenow Fragment still be valid when the length of DNA 3'-overhang is over 10 bases. And what if the 3'-end has been Phosphorylated, does it...

30 December 2015 8,852 1 View