Is that possible to sequence a target directly from extracted genomic DNA (~ 300 Mb) without the previous PCR amplification step?

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Felippe Buck Campana

The genome size is 300 Mb. Obviously I don’t wanna sequence it all! The focus is only ~700 bp after primer site.

Kevin Cavallin

Because of the low target number, it's unlikely that you will get any sequence from a product that large (300 Mb). It would be best if you PCR amplified out that 700 base region before sequencing.