I got into a situation with my manager that compromises my ethical values; none of the options seem particularly great. What can I do?

I got into a situation with my manager that compromises my ethical values; none of the options seem particularly great. What can I do?

01 January 1970 1,731 17 View

What do you feel right after you visited social media? Happy or Sad, and why?

Here is the result from a recent research conducted on screen time!

01 January 1970 5,225 34 View

What are the major commercial Cloud Infrastructure Security Issues?

What are the major commercial Cloud Infrastructure Security Issues? Kindly Share: Research findings, opinions or personal experiences!

01 January 1970 7,246 4 View

What are the benefits of Cloud Computing services to enterprise organizations?

What are the benefits of Cloud Computing services to enterprise organizations?

01 January 1970 4,061 11 View

Automatic data visualization?

Deep eye, a novel automatic data visualization system leverages ML techniques as black-boxes and expert specified rules with promising results. What is your thought on automatic data visualization?

01 January 1970 6,013 8 View

Tableau vs Qlik Sense vs Power BI??

Which do you like to go for your data visualization, Tableau, Qlik Sense or Power BI ? and Why?

01 January 1970 8,173 7 View

Does the food you eat affects your brain? Yes/No and How?

Share Your opinion or any scientific evidence. Have a wonderful discussion forum!

01 January 1970 4,223 40 View

What are your approaches to ethical decision making in the IT context?

To function effectively, professional IT practitioners need not only the appropriate technical knowledge, skills and experience, but also a broad understanding of the context in which they...

01 January 1970 3,628 10 View

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01 January 1970 3,367 19 View

What is tokenization do in security?

Any idea, or thoughts are welcome!

01 January 1970 2,903 20 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

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22 February 2021 9,932 11 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View