The algal cell always gather together when the cells concentration is high and those cells would block the microfluidic chips. How can i handle it?

I want to pump a high concentration algal cells into microfluidic chip, however those cells are always gather together forming a clusters to block the connection between Teflon tube and...

26 October 2020 5,366 2 View

What kind of small mobile robots are available on market to undertake multi-robot research?

Dear All, What kind of small mobile robots are available in market to undertake multi-robot research? Would you have anything to be recommended? Thanks! Chaomin Luo ~~~ Chaomin Luo,...

04 October 2020 5,173 5 View

Why some structural equation models are all observation variables?

Hello everyone. Recently, when I searched the literature about SEM, I found some problems that I couldn't solve. I found that the models established in some studies are all observation variables...

16 July 2020 3,279 3 View

2D protein gel system?

Want to set up a 2D protein gel system. Can anybody recommend a system to go for? We use a lot of Bio-Rad's protein gel systems, but don't know which one is best for 2D gels.

07 July 2020 6,327 3 View

Is there any open source code for learning in the field of data-driven robust optimization?

I can get a lot of theoretical knowledge in related fields, but I'm still confused about the implementation behind it. Unfortunately, I didn't find any examples on the Internet that I could...

29 March 2020 6,438 3 View

Why the large grain contains small grain inside ?

We currently recieved a sample from industry. It is high manganese steel (Hadfield steel) with a composition of Fe-12Mn-1.1C, after austenization treatment. We find that the grain size of this...

15 January 2020 6,624 1 View

How to read the intersection signal information in the Internet of Vehicles environment?

The existing method can be realized by short-distance traffic through the DSRC, or the signal light device can be connected to the roadside device RSU by wire. Even in the driverless field, the...

18 November 2019 6,706 1 View

Are there recommendations to buy some small mobile robots to test our multi-robot algorithms?

We would like to validate and test our multi-robot algorithms with some research-platformed mobile robot. Are there recommendations to buy some small mobile robots to test our multi-robot...

15 November 2019 5,772 3 View

How should the bone model be considered when screw drilling into bone finite element modeling?

For the establishment of bone model, besides considering the bone density, elastic modulus, poisson's ratio, yield stress and yield strain, should the damage caused by bone being cut by screws be...

09 November 2019 5,831 3 View

Why a picture captured from a linear array CCD have a sinusoidal stripe pattern?

We used linear array CCD to capture movement object pictures. When extracting information from the pictured, we were bothered by the intensive sinusoidal stripe pattern (like the files jpg). What...

06 October 2019 5,846 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View