These pictures are with matrigel, E8, on day 2 after thawing. I don't believe it's contamination, but I am surprised that my cell culture still doesn't show healthy confluence. Are these cells dead? Is this a typical step in thawing iPSCs? What could have caused this strange appearance (i.e. pipetting cryo medium + cells into DMEM buffer, insufficient matrigel coating, slow heating of frozen cell sample)?
Hi,
Based on your pics, I can see that your iPSCs have differentiated. I dont see any colonies of iPSCs that could be saved. I think its better to thaw a vial and culture.
They look pretty dead. What kind of media are you using? Usually standard DMEM media isn't compatible with iPSCs. You can also add things like ROCK inhibitor to the media at thawing to improve survival.
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