Is species of the antigens important for immunisation?

oes anyone know if I want to immunize Lewis rats with lacrimal antigens, whether it is important to use lacrimal extract from a different species like SD rats, or even from C57B6 mice?

31 December 2015 3,795 3 View

Question on animal models of disease?

Does anyone have experience doing a delayed hypersensitivity type model in the eye?

31 December 2015 7,682 1 View

What is a good ELISA kit for histamine, tryptase or chymase?

Need to test on tears (small fluid sample)

31 December 2015 5,588 1 View

Would infusion clontech be the best approach for a sequence 4k long for 5race and 3race and cloning?

Thanks in advance.

02 March 2014 5,533 2 View

Can anyone give some guidance on DNA sequencing, reaction and services?

If I have the reverse transcription products from total cellular RNA, is it possible to sequence fragments with specific primers without gel purification of the cdna first?

09 October 2013 5,015 5 View

Does any one know the best place to get hydron pellets for corneal pocket model of angiogenesis?

Seeking advise on animal model

07 August 2013 5,478 0 View

No title

02 March 2021 3,060 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

No title

28 February 2021 9,936 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View