Can I use g_MMPBSA with Cas9?

Hello, I'm trying to see the interaction between the Sa Cas9 protein and the guide RNA. I ran a molecular dynamic simulation on it and then estimated the energy binding using the g_MMPBSA...

17 December 2020 661 2 View

What is the Method of Moment theory and what differentiates it from the MLFMM solver?

Hi everyone, It's been quite a time since I have started searching the web for clear explanations on the Method of Moment (MoM) theory for electromagnetic field computing. Does anyone know a...

23 September 2020 2,763 4 View

What are the latest research in control system ?

Hello all, I want to do my research in control systems (nonlinear, robust, adaptive) and I'm wondering what to study ? Do you guys have any ideas ? I am a bit lost as those fields are...

06 February 2020 8,659 15 View

How to make multivariate time series regression in R?

I m analysing the determinant of economic growth by using time series data. Collected data covers the period from 1980 to 2017. There is one dependent variable and 12 independent variables. The...

06 January 2020 8,411 14 View

Can the bending of a light ray passing the sun correctly be explained by gravitoelectromagnetism?

04 October 2019 9,860 9 View

How to Conduct SPSS Reliability Test For Multi-variable Questionnaire?

Greetings, I am a DBA student conducting a study about "Factors Influencing the Adoption of Payment Cards in Lebanon". My research model, theoretically based on the Technology Adoption Model...

29 January 2019 2,369 7 View

Issues with diagram in AMOS, can anyone help ?

Hello, I'm a student and for my last essay, I have to use structural equation modeling. I'm kind of lost here, and I don't really know what i'm doing on the software, and I have poor statistical...

14 August 2018 8,002 3 View

Has anybody an experience of Fluid exchange interaction into Abaqus CAE Finite Element Package ?

Hello everyone, I am investigating Pressure evolution in response to external solicitation in Finite Element Analysis. I try to iterate my model by enabling a Fluid Exchange interaction between...

14 November 2017 6,973 5 View

How to compute the ionization energy within a frozen solvent cage in PCM

Hi, I'm trying to calculate the IE of a molecule (solute) within a solvent. It seems like I can get a fair approximation using PCM solvation model, however I do not want to let the environment...

30 July 2017 8,247 3 View

Where does this information come from?

from uniprot : Nedd4 binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif.

25 July 2017 9,694 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View