I'd like to use Lrg5 mice to trace stem cells in the skin
is it useful to use Lrg5 to mark stem cells in the skin as I read that it mark stem cells in the small intestine
I am gonna use Resiniferatoxin (RTX) in my experiment found that it is a powder(1 &5 mg) that needed to be dissolved in a vehicle to be allowed for injection when I checked some papers I...
05 June 2019 4,898 2 View
I used 6-OH-dopamine to induce sympathetic denervation in mice How long I should use it Is their any clear signs for denervation or as reviews mentioned to use it for 7 to 14 days
03 April 2019 697 2 View
I got different ways from YouTube to measure fluorescent intensity and some of them not totally clear. So I would be appreciated if someone explain to me how to do it ?
02 March 2019 9,670 2 View
what is the degree of immunofluorescence color showing p53 in stem cells than others? also is it concentrated inside the nucleus or cytoplasm or both?
01 February 2019 5,040 2 View
I'd like to know how many cells I need to get 1 ug nuclear extraction? Can someone help me with the protocol for nuclear extraction
31 December 2018 4,089 0 View
I still have amplified of my target DNA and like to run them on jel again the DNA amplified from a month ago and stored in the fridge 4C. is it still possible to use them again for running jel?
09 October 2018 2,050 5 View
If I have samples (tails) of unknown kind of mice but the mice should be either transgenic (heterozygous) or not (wild type I am now have a forward primer and 2 reverse primer one mutant...
08 September 2018 2,280 2 View
How can we draw the lineage tress ? did we use a certain software or just using excel and word aatached is a sample fig from a paper to Amit Roshan et al., " Human keratinocytes have two...
07 August 2018 8,379 0 View
I have a lypholized primer ( powder) 15.51 nmol 95.05 ug 3.25 OD 6124.99 MW I'd like to dilute it to initial concentration 10 uM volume/reaction 0.5 ul final concentration 5uM...
07 August 2018 4,073 6 View
Hello every body, I performed a study test in the mouse wounds and I used the punch skin biopsy to create wounds on the mouse skin. I'd like to know the best way to cover the wounds. I used the...
06 July 2018 4,052 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...
30 July 2024 9,806 1 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View