How to dissolve RTX?

05 June 2019 4,814 2 View

How can we evaluate physically the effect of Sympathetic denervation in mice?

I used 6-OH-dopamine to induce sympathetic denervation in mice How long I should use it Is their any clear signs for denervation or as reviews mentioned to use it for 7 to 14 days

03 April 2019 609 2 View

Could any body please guide me how to use image J to measure color intensity? ?

I got different ways from YouTube to measure fluorescent intensity and some of them not totally clear. So I would be appreciated if someone explain to me how to do it ?

02 March 2019 9,589 2 View

What is the expression of P53 in stem cells than other cells?

what is the degree of immunofluorescence color showing p53 in stem cells than others? also is it concentrated inside the nucleus or cytoplasm or both?

01 February 2019 4,974 2 View

How many cells we need to get 1 ug nuclear extract?

I'd like to know how many cells I need to get 1 ug nuclear extraction? Can someone help me with the protocol for nuclear extraction

31 December 2018 4,025 0 View

How long the amplified DNA be valid for electrophoresis?

I still have amplified of my target DNA and like to run them on jel again the DNA amplified from a month ago and stored in the fridge 4C. is it still possible to use them again for running jel?

09 October 2018 1,973 5 View

Which primer I should use in master mix for unknown sample?

If I have samples (tails) of unknown kind of mice but the mice should be either transgenic (heterozygous) or not (wild type I am now have a forward primer and 2 reverse primer one mutant...

08 September 2018 2,210 2 View

Is there any body could kindly help and guide me how can i draw a lineage trees for cultured cells?

How can we draw the lineage tress ? did we use a certain software or just using excel and word aatached is a sample fig from a paper to Amit Roshan et al., " Human keratinocytes have two...

07 August 2018 8,251 0 View

What is the best way to cover the wounds in mice?

Hello every body, I performed a study test in the mouse wounds and I used the punch skin biopsy to create wounds on the mouse skin. I'd like to know the best way to cover the wounds. I used the...

06 July 2018 3,978 3 View

Which is the Forward primer and which is the reverse primer?

Please can any body help me how to know the forward and reverse Primer I have a transgenic mice and I breeded them with wild type and I'll make a genotyping for the progeny to detect the...

06 July 2018 9,036 3 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View