What is the expression of P53 in stem cells than other cells?

More Ayman Khalifa's questions See All

How to dissolve RTX?

I am gonna use Resiniferatoxin (RTX) in my experiment found that it is a powder(1 &5 mg) that needed to be dissolved in a vehicle to be allowed for injection when I checked some papers I...

05 June 2019 4,898 2 View

How can we evaluate physically the effect of Sympathetic denervation in mice?

I used 6-OH-dopamine to induce sympathetic denervation in mice How long I should use it Is their any clear signs for denervation or as reviews mentioned to use it for 7 to 14 days

03 April 2019 697 2 View

Could any body please guide me how to use image J to measure color intensity? ?

I got different ways from YouTube to measure fluorescent intensity and some of them not totally clear. So I would be appreciated if someone explain to me how to do it ?

02 March 2019 9,670 2 View

How many cells we need to get 1 ug nuclear extract?

I'd like to know how many cells I need to get 1 ug nuclear extraction? Can someone help me with the protocol for nuclear extraction

31 December 2018 4,089 0 View

How long the amplified DNA be valid for electrophoresis?

I still have amplified of my target DNA and like to run them on jel again the DNA amplified from a month ago and stored in the fridge 4C. is it still possible to use them again for running jel?

09 October 2018 2,050 5 View

Which primer I should use in master mix for unknown sample?

If I have samples (tails) of unknown kind of mice but the mice should be either transgenic (heterozygous) or not (wild type I am now have a forward primer and 2 reverse primer one mutant...

08 September 2018 2,280 2 View

Is there any body could kindly help and guide me how can i draw a lineage trees for cultured cells?

How can we draw the lineage tress ? did we use a certain software or just using excel and word aatached is a sample fig from a paper to Amit Roshan et al., " Human keratinocytes have two...

07 August 2018 8,379 0 View

Could any body kindly please help me how to make a primer dilution?

I have a lypholized primer ( powder) 15.51 nmol 95.05 ug 3.25 OD 6124.99 MW I'd like to dilute it to initial concentration 10 uM volume/reaction 0.5 ul final concentration 5uM...

07 August 2018 4,073 6 View

What is the best way to cover the wounds in mice?

Hello every body, I performed a study test in the mouse wounds and I used the punch skin biopsy to create wounds on the mouse skin. I'd like to know the best way to cover the wounds. I used the...

06 July 2018 4,052 3 View

Which is the Forward primer and which is the reverse primer?

Please can any body help me how to know the forward and reverse Primer I have a transgenic mice and I breeded them with wild type and I'll make a genotyping for the progeny to detect the...

06 July 2018 9,187 3 View

Is skin yellowness an numerical or ordinal variable?

I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...

11 August 2024 4,793 1 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Why don't d-orbitals split themselves, why does it take a ligand? why don't protons from ligand repel nucleus split d-orbitals?

why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...

03 August 2024 3,589 3 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View