I'd like to know how many cells I need to get 1 ug nuclear extraction?
Can someone help me with the protocol for nuclear extraction
I am gonna use Resiniferatoxin (RTX) in my experiment found that it is a powder(1 &5 mg) that needed to be dissolved in a vehicle to be allowed for injection when I checked some papers I...
05 June 2019 4,898 2 View
I used 6-OH-dopamine to induce sympathetic denervation in mice How long I should use it Is their any clear signs for denervation or as reviews mentioned to use it for 7 to 14 days
03 April 2019 697 2 View
I got different ways from YouTube to measure fluorescent intensity and some of them not totally clear. So I would be appreciated if someone explain to me how to do it ?
02 March 2019 9,670 2 View
what is the degree of immunofluorescence color showing p53 in stem cells than others? also is it concentrated inside the nucleus or cytoplasm or both?
01 February 2019 5,040 2 View
I still have amplified of my target DNA and like to run them on jel again the DNA amplified from a month ago and stored in the fridge 4C. is it still possible to use them again for running jel?
09 October 2018 2,050 5 View
If I have samples (tails) of unknown kind of mice but the mice should be either transgenic (heterozygous) or not (wild type I am now have a forward primer and 2 reverse primer one mutant...
08 September 2018 2,280 2 View
How can we draw the lineage tress ? did we use a certain software or just using excel and word aatached is a sample fig from a paper to Amit Roshan et al., " Human keratinocytes have two...
07 August 2018 8,379 0 View
I have a lypholized primer ( powder) 15.51 nmol 95.05 ug 3.25 OD 6124.99 MW I'd like to dilute it to initial concentration 10 uM volume/reaction 0.5 ul final concentration 5uM...
07 August 2018 4,073 6 View
Hello every body, I performed a study test in the mouse wounds and I used the punch skin biopsy to create wounds on the mouse skin. I'd like to know the best way to cover the wounds. I used the...
06 July 2018 4,052 3 View
Please can any body help me how to know the forward and reverse Primer I have a transgenic mice and I breeded them with wild type and I'll make a genotyping for the progeny to detect the...
06 July 2018 9,187 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...
01 August 2024 9,993 3 View