How to dissolve RTX?

05 June 2019 4,814 2 View

How can we evaluate physically the effect of Sympathetic denervation in mice?

I used 6-OH-dopamine to induce sympathetic denervation in mice How long I should use it Is their any clear signs for denervation or as reviews mentioned to use it for 7 to 14 days

03 April 2019 609 2 View

Could any body please guide me how to use image J to measure color intensity? ?

I got different ways from YouTube to measure fluorescent intensity and some of them not totally clear. So I would be appreciated if someone explain to me how to do it ?

02 March 2019 9,589 2 View

What is the expression of P53 in stem cells than other cells?

what is the degree of immunofluorescence color showing p53 in stem cells than others? also is it concentrated inside the nucleus or cytoplasm or both?

01 February 2019 4,974 2 View

How long the amplified DNA be valid for electrophoresis?

I still have amplified of my target DNA and like to run them on jel again the DNA amplified from a month ago and stored in the fridge 4C. is it still possible to use them again for running jel?

09 October 2018 1,973 5 View

Which primer I should use in master mix for unknown sample?

If I have samples (tails) of unknown kind of mice but the mice should be either transgenic (heterozygous) or not (wild type I am now have a forward primer and 2 reverse primer one mutant...

08 September 2018 2,210 2 View

Is there any body could kindly help and guide me how can i draw a lineage trees for cultured cells?

How can we draw the lineage tress ? did we use a certain software or just using excel and word aatached is a sample fig from a paper to Amit Roshan et al., " Human keratinocytes have two...

07 August 2018 8,251 0 View

Could any body kindly please help me how to make a primer dilution?

I have a lypholized primer ( powder) 15.51 nmol 95.05 ug 3.25 OD 6124.99 MW I'd like to dilute it to initial concentration 10 uM volume/reaction 0.5 ul final concentration 5uM...

07 August 2018 4,001 6 View

What is the best way to cover the wounds in mice?

Hello every body, I performed a study test in the mouse wounds and I used the punch skin biopsy to create wounds on the mouse skin. I'd like to know the best way to cover the wounds. I used the...

06 July 2018 3,978 3 View

Which is the Forward primer and which is the reverse primer?

Please can any body help me how to know the forward and reverse Primer I have a transgenic mice and I breeded them with wild type and I'll make a genotyping for the progeny to detect the...

06 July 2018 9,036 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View