17 Questions 17 Answers 0 Followers
Questions related from Ayman Khalifa
I am gonna use Resiniferatoxin (RTX) in my experiment found that it is a powder(1 &5 mg) that needed to be dissolved in a vehicle to be allowed for injection when I checked some papers I...
06 June 2019 4,872 2 View
I used 6-OH-dopamine to induce sympathetic denervation in mice How long I should use it Is their any clear signs for denervation or as reviews mentioned to use it for 7 to 14 days
04 April 2019 673 2 View
I got different ways from YouTube to measure fluorescent intensity and some of them not totally clear. So I would be appreciated if someone explain to me how to do it ?
03 March 2019 9,641 2 View
what is the degree of immunofluorescence color showing p53 in stem cells than others? also is it concentrated inside the nucleus or cytoplasm or both?
02 February 2019 5,017 2 View
I'd like to know how many cells I need to get 1 ug nuclear extraction? Can someone help me with the protocol for nuclear extraction
01 January 2019 4,069 0 View
I still have amplified of my target DNA and like to run them on jel again the DNA amplified from a month ago and stored in the fridge 4C. is it still possible to use them again for running jel?
10 October 2018 2,028 5 View
If I have samples (tails) of unknown kind of mice but the mice should be either transgenic (heterozygous) or not (wild type I am now have a forward primer and 2 reverse primer one mutant...
09 September 2018 2,258 2 View
I have a lypholized primer ( powder) 15.51 nmol 95.05 ug 3.25 OD 6124.99 MW I'd like to dilute it to initial concentration 10 uM volume/reaction 0.5 ul final concentration 5uM...
08 August 2018 4,053 6 View
How can we draw the lineage tress ? did we use a certain software or just using excel and word aatached is a sample fig from a paper to Amit Roshan et al., " Human keratinocytes have two...
08 August 2018 8,345 0 View
Please can any body help me how to know the forward and reverse Primer I have a transgenic mice and I breeded them with wild type and I'll make a genotyping for the progeny to detect the...
07 July 2018 9,147 3 View
Hello every body, I performed a study test in the mouse wounds and I used the punch skin biopsy to create wounds on the mouse skin. I'd like to know the best way to cover the wounds. I used the...
07 July 2018 4,031 3 View
Hello every body, could any body kindly support me about the data of the subcutaneous injection of Sonic hedgehog to mice to improve wound healing what is the recommended dose and the protocol...
06 June 2018 4,934 2 View
I am going to use a chemical and I found its dose to the rats and it is not clear for the mouse can I use the same dose as it depends on weight?
06 June 2018 1,781 4 View
Hello every body, I am going to use Numa and Tubulin to detect the cell divisions ( almost during meta-phase stage) in mice skin I tried alpha Tubulin Monoclonal Antibody (TU-01) as a tubule...
06 June 2018 4,100 4 View
I am very depressed as I got poor results with NUMA and Tubulin I tried to use 2 different kinds of both antibodies and for several times but unfortunately I got a poor results. I dont know where...
06 June 2018 4,925 0 View
I'd like to use Lrg5 mice to trace stem cells in the skin is it useful to use Lrg5 to mark stem cells in the skin as I read that it mark stem cells in the small intestine
04 April 2018 368 0 View
please can I use this mice (Lgr5-EGFP-IRES-creERT2) to mark the stem cells in the skin?
04 April 2018 7,105 0 View
