is Lactobacillus MRS agar used to isolate all Lactic acid bacteria? agan i need to supplement 0.3% of Calcium Carbonate to MRS agar, how can i supllemnt , before or after sterilization( autoclaving) the media?
Thank you
MRS agar normally used to isolate lactobacilus or all type of LAB, you can add specific nutrients if you want to isolate specific LAB, calcium carbonate can be added before autoclaving the media, but I usually add bromocresol purple into media, which is the same thing.. regards
Dear
MRS media is selective media for Lactobacilli but some bacteria can grow on MRS such as species of enterobacteraceae. Adding CaCo3 is increasing selective of media. The CaCo3 before autoclaving process. I suggest you , the bacteria growth is under anaerobic condition.
MRS agar is not a selective media for lactobacillus sp. It grow all lactic acid bacteria. Mrs agar supplement with calcium carbonate - 0.5%, sodium azide- 10mg/L, cycloheximide 1mg/ml under anaerobic condition for 37 degree Celsius at 24 h and appeared clear zone on background of plate.
MRS agar is not a selective media for lactobacillus sp. It grow all lactic acid bacteria.
this link is very useful for you for this subject
http://ori-oai.u-bordeaux1.fr/pdf/2010/DALIE_DOGUIET_2010.pdf
But today modified MRS agar available in the Himedia and they also given indicator (bromocresol blue and cycloheximide) i.e. only for specific isolation of lactobacillus
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