Is it safe to use Insulin detemir in pregnant woman?

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I would like to quantitatively detect total bacteria in mice feces. How can I obtain a standard curve to reveal total bacteria quantitatively?

Basically, I would like to quantitatively detect total bacteria in mice feces. How can I obtain a standard curve to reveal total bacteria quantitatively? By the way, I have one bacterial species...

23 January 2024 9,254 2 View

How can I identify a bacterium?

Hello everyone, I have the complete genome sequence of a bacterium in an assembly form. How can I easily and practically determine which strain it is? Also, how can I find the 16S rRNA of this...

10 December 2022 6,965 3 View

How do I calculate the protein content from absorbance and standard curve and express as g/100g or percentage?

I did bradford assay and I want to calculate the amount of soluble protein in my glycoprotein sample. I prepared BSA stock of 1mg/ml and did a standard curve ranging from 0.01 to 0.16 mg/ml. I...

06 December 2022 604 0 View

What is the storage condition of freshly prepared OPA reagent?

How do you store OPA reagent made from scratch and how long can it be stored for use?

22 March 2022 1,655 2 View

Do the attached photos show contamination in the cell culture study?

While our studies were going on, we noticed long thin formations around the cells as in the photo. While trying to understand what was happening, we saw that it was also in the newly opened...

03 January 2021 6,493 1 View

How to prepare 4% PFA/PBS for zebrafish embryo ALP staining?

I want to prepare fixation buffer 4% PFA/PBS but I'm confused with the methods I've come across. I tried to follow this one method whereby I dissolved 4g of PFA (merck, for synthesis) in 50 ml of...

09 July 2017 8,287 5 View

Would you like to ask you kindly to enhance enzymatic saccharification on woody-biomass?

My study is based on pretreatments and enzymatic saccharification to convert cellulose to glucose in lignocellulosic biomass. I tried several pretreatments which are liquid hot water, very dilute...

21 March 2016 1,342 4 View

Is PHENOMENEX KJ0-4282 Security Guard really a universal HPLC guard cartridge and can it fit all types of HPLC columns?

PHENOMENEX KJ0-4282 Security Guard is said to be a universal HPLC gurad cartridge for analytical columns. However, I have problems when installing it to a WATERS XBridge C18 column 5 μm 4.6 x...

02 September 2015 3,593 10 View

Should I ignore an initial burst for drug release while examining the appropriate drug release model?

Just between zero, first order, Huguchi and Peppas models.

07 January 2015 1,654 3 View

Any suggestions for a work up for 2-Amino-1-(4-nitrophenyl)-1,3-propanediol after deamidation from chloramphenicol?

I have prepared 2-Amino-1-(4-nitrophenyl)-1,3-propanediol by acid catalysed deamidation of chloramphenicol and confirmed the structure by LC-MS. I need to purify the product and determine its...

29 January 2014 2,592 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

Does exposure to toxins during pregnancy increase early puberty risk?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 8,361 0 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View