What can be the maximum Tm of a TaqMan probe? How to avoid nonspecific binding of a probe if there is 75% similarity in DNA sequence was to be found?

I'm designing species specific PCR probe (DNA hybridization probe) for a single gene in 10 different species as a diagnosis tool. I'm able to find DNA regions which are unique for the particular...

26 June 2020 9,278 0 View