We have an MS detector for GC in our laboratory. I want to know whether we can use this detector to couple the LC system.
you can coupled GC and HPLC, but if you have to know the intrrumentations to connect two computers working as a programming will be carried out on MS,
GC-MS and LC-MS typically use totally different mechanisms for ionisation. YOU CANT USE A MS OF GC TO HPLC
GC/MS requires the analyte to be vaporized in order for migration through the capillary to occur. Analytes, therefore, must be volatile or amenable to chemical derivatization torender them volatile.Certain types of samples are particularly well suited to GC/MS analyses These include plant terpenes and essential oils, which are volatile and do not ionize well by LC/MS techniques. After derivatization, free fatty acids are also amenable to GC/MS. The high chromatographic resolution of GC
permits separation of structurally similar fatty acids that would be very difficult to
separate by HPLC. GC/MS provides greater sensitivity than LC/MS for free
fatty acids. Other analytes generally compatible with GC/MS include steroids, diglycerides, mono-, di- and tri-saccharides, and sugar alcohols.
Liquid chromatography can separate metabolites that are not volatile and
have not been derivatized. As a result, LC/MS can analyze a much wider range of chemical species than GC/MS. Samples commonly analyzed by LC/MS
include amino acids (18 out of 20 amino acids can be derivatized, but the
remaining two can not) and sugars larger than trimers.
Actually the answer is maybe rather than no. It depends on the ion sources that are available for your MS. GC produces a gaseous effluent, which can ionized by Electron Impact (EI). The effluent from the LC is liquid, which can be ionized by Electrospray Ionization (ESI) or, if you want to look for the more generic term, API (atmospheric pressure ionization). If you have both sources and can swap in a straightforward manner than it might be possible.
Yes, with the proper interface. The detector for a particle beam interface is essentially that of a GC-MS (the first one WAS a MS used with a GC, since Browner pulled his GC off of his 5985).
The assumption that you must ionize prior to desolvation is a very poor assumption. There are multiple ionization techniques available; just because some of them are not readily commercially available does NOT mean that they do not work.
If you build or purchase a particle beam interface (www.cssco.com) you will be able to seamlessly use your LC with your existing MS.
Dear Esmaeel,
It would be easy to say "no", but for now some instruments can be coupled with LC and GC. But you should know, that you can't use electron ionization in LC! Just look at Waters Atmospheric Pressure Gas Chromatography (APGC). This is just ion source. You can change it to the ESI or APCI and work with LC.
P.S. I hope this link will be usefull for you.
Best wishes,
Azamat
In normal practice there is no possible to couple directly MS detector (from GC/MS system) with HPLC. One of the reasons is the particle beam interface and the mode of ionization of the liquid sample by use Electrospray Ionization (ESI) instead of Electron Impact Ionisation (EI) mode.
- Badges
- Science method