Is it possible whole organ regeneration from decellularized organ in vitro?

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Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,138 2 View

Can sulfuric acid be used to prepare anthrone reagent when it has oxidized and changed color in reaction to light?

Sulfuric acid has changed color to yellow.

16 July 2024 6,964 0 View

What is the suitable freezing media and protocol for human mature adipocytes cryopreservation?

I used regular freezing media (90% FBS and 10% DMSO) for cryopreservation of isolated human mature adipocytes from adipose tissue in -80 degree. When I freezed the cells, the number of cell was...

26 May 2024 7,803 0 View

How it is possible to analyze human mature adipocytes cell markers with flow cytometry? what are the essential optimizations?

As human adipocytes can be up to 300 μm in diameter, How these cells can be analyzed for cell markers by flow cytometry with 100 micron Nozzles? What changes are necessary to be done on flow...

12 May 2024 2,254 0 View

Gel Electrophoresis Troubleshooting?

Hello everyone, in the gel results image provided, the samples in lanes 2 and 5 represent linearized plasmid DNA from groups A and B respectively. Additionally, the samples in lanes 3 and 6 depict...

25 April 2024 7,126 1 View

How can i remove oil layer from adipocyte layer in human mature adipocyte isolation?

I want to isolate adipocytes from human adipose tissue. but after isolation and culture them, there were very larg droplets between small to moderate size cells in 12-wells plate that seemingly...

20 March 2024 5,945 0 View

I want to adhere mature human adipocytes to bottom of Petri dishes or flasks for cell culture. Which coated material or method do you recommend?

After mature adipocytes isolation from human adipose tissue, I want to adhere them for my studies (Adipocytes tend to suspend on top layer of the media). Which method or materials do you...

06 March 2024 903 1 View

How can I establish a bond (amine and carboxylic group) between plga and peg?

Hello, how can I establish a bond (amine and carboxylic group) between PLGA and PEG as a drug carrier? What is the problem with my work? please guide me The mixture containing PEG,...

18 January 2024 9,737 0 View

Why does the coercivity of cobalt ferrite decrease at low temperature 70k?

Following diagram show Hysteresis loops of CoFe2O4 Spherical nanoparticles at 300k & 70K (liquid Nitrogen). Why such decrease in hysteresis loop and Hc have been seen with reduce temperature?

13 August 2023 1,275 5 View

How to remove lime deposited on pottery without ruining pottery and painted motives on it?

I have some pottery sherds with significant motives on them, but unfortunately, lime covered most of them, is there any way to remove the lime without damaging the pottery surface or painted motives?

24 July 2023 2,986 0 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,283 4 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Arash Abdolmaleki

Dear Dr

See the following manuscript

Article Refunctionalization of Decellularized Organ Scaffold of Panc...

Aref Wazwaz

Dear Dr Asadollah Asadi . See the following useful RG link: Article Strategies based on organ decellularization and recellularization

Kindly see also the following useful RG link: Article Whole organ regeneration: an overview of the recent advances...

Also check please the following very good RG link: Article Coming to TERMs with Tissue Engineering and Regenerative Med...

The following RG link is very useful: Book Decellularization Methods of Tissue and Whole Organ in Tissu...

Asadollah Asadi

Thank you Dear Aref.