Can SiO2 deposition be used to protect gold electrodes (in direct contact with ionic solution) from electrolyis?

Will the electrodes remain conductive? What should be the thickness of SiO2 deposition?

03 April 2018 2,919 3 View

How can I protect thin film (30nm) gold electrodes from electrolysis?

I apply voltage (AC, DC and pulse) to biological cell monolayers using 30nm thick gold electrodes. My observations are significantly affected by electrolysis (formation of gas bubbles). Moreover,...

01 February 2018 7,950 6 View

When electrolysis occurs, does the anode or cathode heat up more (or by same extent)?

Does it depend on the relative size of the electrodes?

01 February 2018 9,611 4 View

When doing biological cell migration, can electrode be connected directly to agar bridge, or does it need to be immersed in salt solution?

Does the electrode need to be of a specific metal? Which salt solution and concentration to use?

09 October 2017 6,779 1 View

Can a gold pattern on glass slide work as inductive coil?

If so, what is the most appropriate pattern shape and gold thickness?

06 July 2017 431 3 View

Is it possible to fabricate gold electrodes on pdms? what adhesion layer or pdms surface treatment is required?

will the electrodes withstand bending?

01 February 2017 8,921 1 View

Is there a reliable way of "visualising" an electric field between 2 planar electrodes?

My electrodes are 2 bands of gold with a gap in between || Is there any way of making sure an electric field exists between the electrodes?

09 October 2016 3,490 6 View

What is the physiological shear stress on mouse endothelial cells?

i would like to carry out flow experiments to determine how culture medium flow affects cell morphology

01 January 1970 6,654 4 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View