I wish to determine live/dead cell ratio while the cells are still in a monolayer
Naturally it IS possible to stain a cell monolayer with trypanblue/ to use TB to stain . You should specify your question to get a sufficient answer.....
Will the electrodes remain conductive? What should be the thickness of SiO2 deposition?
03 April 2018 3,009 3 View
Does it depend on the relative size of the electrodes?
01 February 2018 9,696 4 View
I apply voltage (AC, DC and pulse) to biological cell monolayers using 30nm thick gold electrodes. My observations are significantly affected by electrolysis (formation of gas bubbles). Moreover,...
01 February 2018 8,075 6 View
Does the electrode need to be of a specific metal? Which salt solution and concentration to use?
09 October 2017 6,861 1 View
If so, what is the most appropriate pattern shape and gold thickness?
06 July 2017 539 3 View
will the electrodes withstand bending?
01 February 2017 8,992 1 View
My electrodes are 2 bands of gold with a gap in between || Is there any way of making sure an electric field exists between the electrodes?
09 October 2016 3,576 6 View
i would like to carry out flow experiments to determine how culture medium flow affects cell morphology
01 January 1970 6,742 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?
29 July 2024 7,404 1 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View