Hello, 

I would like to know if it is possible to use S1 nuclease instead of P1 nuclease for gDNA digestion, prior to LC-ESI-MS/MS genome methylation determination?

Here is the base article: 

Song, L., James, S. R., Kazim, L., & Karpf, A. R. (2005). Specific Method for the Determination of Genomic DNA Methylation by Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry. Analytical Chemistry, 77(2), 504-510. https://doi.org/10.1021/ac0489420

Thank you for your answers!