To evaluate the efficiency of each type of bacteria
life is full of the bad and sad news that may affect people life
04 May 2019 5,247 4 View
Boiling method is one of the simplest methods of DNA extraction, is it sufficient for the different applications of biotechnology
09 October 2018 771 0 View
DNA consist of series of regions coded for the whole genome.
05 June 2018 5,022 0 View
Data for DNA sequencing that taking from organisms need to use computers to determine the differences between these organisms.
05 June 2018 5,222 0 View
There is a process used for synthesis oligonucleotides on the glass slide
05 June 2018 6,462 1 View
every somatic cell in the body has basically the same DNA.
04 May 2018 1,537 0 View
Isoamyl alcohol is one of several chemical materials used in the extraction of DNA.
03 April 2018 8,853 2 View
chloroform is one of several chemical materials used in the extraction of DNA.
03 April 2018 3,713 2 View
one of the important steps in the extraction of DNA we use ethanol.
03 April 2018 3,969 0 View
In the process of DNA extraction, we use proteinase K.
02 March 2018 4,725 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
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There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
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I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
