To evaluate the efficiency of each type of bacteria
life is full of the bad and sad news that may affect people life
04 May 2019 4,952 4 View
Boiling method is one of the simplest methods of DNA extraction, is it sufficient for the different applications of biotechnology
09 October 2018 705 0 View
DNA consist of series of regions coded for the whole genome.
05 June 2018 4,777 0 View
There is a process used for synthesis oligonucleotides on the glass slide
05 June 2018 6,207 1 View
Data for DNA sequencing that taking from organisms need to use computers to determine the differences between these organisms.
05 June 2018 4,974 0 View
every somatic cell in the body has basically the same DNA.
04 May 2018 1,441 0 View
chloroform is one of several chemical materials used in the extraction of DNA.
03 April 2018 3,370 2 View
Isoamyl alcohol is one of several chemical materials used in the extraction of DNA.
03 April 2018 8,552 2 View
one of the important steps in the extraction of DNA we use ethanol.
03 April 2018 3,720 0 View
In the process of DNA extraction, we use proteinase K.
02 March 2018 4,461 5 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
