I wish to observe the mobility of EPS, and hence to tag or mark the EPS with some fluorescent dye.
I use Pierce assay for protein estimation using a spectrometer and want to try using nanodrop for the same to increase the efficiency and use less amount of sample.
04 May 2018 2,566 6 View
I have biofilms growing on glass beads and I want to measure biofilm thickness.
04 May 2014 8,159 4 View
Normally the method is that the final concentration of Rose Bengal should be 20mg/L which is too dark for a proper UV spc reading. Also, I could not determine a linear region for quantification of...
02 March 2014 6,709 0 View
I want to measure the Vt for my nanoparticles and comment on their stability, but I do not understand how exactly to calculate it Can someone help me with this?
09 October 2012 9,508 2 View
Can any one help me with synthesis of nanosized colloidal Mnoxide? I tried by my oxides precipitate and aggregate after dialysis! Any solutions?
01 February 2012 1,082 1 View
I wish to detect the presence of bactreial EPS and other prodcuts of microbial origin like surfactants from the water sample. Can any one help me???
05 June 2011 2,806 1 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...
24 February 2021 9,210 1 View
Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...
19 February 2021 472 5 View
Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...
17 February 2021 5,399 1 View
I am looking for PCR primers for bacillus Sp.
15 February 2021 2,552 5 View