I wish to observe the mobility of EPS, and hence to tag or mark the EPS with some fluorescent dye.
I use Pierce assay for protein estimation using a spectrometer and want to try using nanodrop for the same to increase the efficiency and use less amount of sample.
04 May 2018 2,633 6 View
I have biofilms growing on glass beads and I want to measure biofilm thickness.
04 May 2014 8,211 4 View
Normally the method is that the final concentration of Rose Bengal should be 20mg/L which is too dark for a proper UV spc reading. Also, I could not determine a linear region for quantification of...
02 March 2014 6,785 0 View
I want to measure the Vt for my nanoparticles and comment on their stability, but I do not understand how exactly to calculate it Can someone help me with this?
09 October 2012 9,572 2 View
Can any one help me with synthesis of nanosized colloidal Mnoxide? I tried by my oxides precipitate and aggregate after dialysis! Any solutions?
01 February 2012 1,140 1 View
I wish to detect the presence of bactreial EPS and other prodcuts of microbial origin like surfactants from the water sample. Can any one help me???
05 June 2011 2,867 1 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...
19 July 2024 4,868 1 View
These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..
13 July 2024 9,701 1 View
I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...
11 July 2024 7,847 1 View
The harmful Dye pollution from industrial processes such as textile generated how to remove with magnetic biochar ?
07 July 2024 8,118 1 View
Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...
07 July 2024 8,200 2 View