Is the test of the H1 hypothesis in mediation analysis total effect or direct effect?

There are three hypotheses and I carry out direct effect and indirect effects with SEM. H1: There is a significant positive relationship between A and B. H2: There is a significant positive...

08 February 2021 9,141 3 View

Can I publish my published dataset (data-report article) later as an original article?

I want to quickly publish a data-report .Because there is a lot of data and it will take time to create the original article. But my main goal is to use that data-set in the original article. Is...

02 February 2021 9,779 7 View

What test(s) do you recommend for non-parametric binary data?

We are working on a study where we compare pre and post-workshop answers from a group of subjects. Subjects answered a question before the workshop and answered the same question after the...

17 January 2021 2,844 6 View

Do you have any practical methods of dipping the same amount of leads into the cell as a working electrode each time?

Dear colleagues, What kind of way do you follow to immerse the same amount of heads as pencil graphite electrodes into the cell every time for the cyclic voltammetry in sensor studies? May you...

18 October 2020 6,045 4 View

Could the relationship between social media use and 'cocooning at home' be investigated in the Covid 19 process?

Dear academics, I want to do a quantitative research on the use of social media in the process of closing the house in Covid 19 process. At this point, 'cocooning', that is, maintaining a...

24 March 2020 3,879 3 View

The standard error in the standardized model results section in Mplus7 does not show the standardized error, t value and p-value?

I'm doing bootstrap 2000 for multiple mediated analysis on Mplus7. However, in the standardized model results section,does not show the standardized error, t value (EST./S.E.) and p-value. How...

17 March 2020 9,102 3 View

No title

09 December 2019 706 3 View

Does anyone have any suggestions about migration, acculturation and consumer behaviour for a research topic?

Migration, Acculturation and Consumer Behaviour

18 October 2018 9,343 5 View

Is it possible to run different control samples in one QPCR for Copy Number Variation?

Hi All, I have checked the related discussions but could not find my answer. I will do mtDNA copy number with using a nuclear gene which is a single copy to compare with a mitochondrial gene whose...

05 October 2018 451 3 View

Granular-like structures in embryonic fibroblast culture?

Hi All, I see some big regions in embryonic fibroblast culture (red circles in the image), I am very familiar with cells, I marked nuclei with blue circle. These can be dying cells or yeast...

18 June 2018 6,388 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View