Is it possible to sequence mitochondrial DNA and genomic DNA simultaneously (at a run)?

More Snijesh V.P.'s questions See All

What is the possibility of replicates being different from other replicates of same sample?

I have analyzed the dataset of GSE38132 from gene expression omnibus. The data is from cell line breast cancer ZR-75-1 which comprises of 9 conditions with 4 replicates for each condition making...

01 February 2020 8,693 4 View

How to perform normalization and calculate when there are multiple groups ?

I have 4 sample [control, pre-treatment, post-treatment, metastais] with 4 replicates which make total 4x4 = 16 samples I want to perform comparison of control with all other groups. For the said...

01 February 2020 5,334 2 View

How does batch correction of gene expression data affect accuracy?

I have two batches of samples which were collected during two time period. If I perform batch correction to remove batch effect will it affect the downstream analysis of gene expression studies?

11 December 2019 1,463 3 View

Does latest version of dbSNP build 151 have all 'rsids' (SNP ids)?

dbSNP has got different build (140 to 151). Does the latest version dbSNP build 151 have all snp ids (rsids) that are present in previous build

06 July 2018 4,809 0 View

Is it possible to get odds ratio of a snp from clinvar database?

Some database like ebi gwas provides odds ratio for snps. I wanted to know whether clinvar database provides the same?. I have whole data of clinvar in excel but I can not find odds ratio in it.

05 June 2018 9,782 2 View

Is combined approach from different research paper a novel methodology?

Consider, 4 research paper with different methodologies to prioritize genes. Combining these methods to single pipeline, can we call it as a "novel method" as together they can not be found in any...

01 February 2018 10,168 2 View

What are the best tools for Data Mining Techniques for Harvesting Data from the Internet?

I want to extract the data from the internet with specific medical terms. Which are the available tools with best accuracy or output?

01 February 2018 9,939 7 View

What is the present status of Next Generation sequencing in Clinical Diagnosis?

What is the present status of Next Generation sequencing in Clinical Diagnosis?. Is it well defined to make a conclusion for diagnosis?.

10 November 2017 9,056 3 View

Organs and part of organ classification?

Hi, I think there are about 80 organs in the human body. When I searched for list of all organs different sites are showing different list. Are there any books or publication that clearly mentions...

10 November 2017 1,146 5 View

RNA-Seq analysis cummerbund package in R?

I performed RNA-Seq data analysis using protocol given in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334321/. When I am using cummerbund package, it shows output like this: CuffSet instance...

07 August 2017 7,291 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View