How to perform normalization and calculate when there are multiple groups ?

More Snijesh V.P.'s questions See All

What is the possibility of replicates being different from other replicates of same sample?

I have analyzed the dataset of GSE38132 from gene expression omnibus. The data is from cell line breast cancer ZR-75-1 which comprises of 9 conditions with 4 replicates for each condition making...

01 February 2020 8,505 4 View

How does batch correction of gene expression data affect accuracy?

I have two batches of samples which were collected during two time period. If I perform batch correction to remove batch effect will it affect the downstream analysis of gene expression studies?

11 December 2019 1,272 3 View

Does latest version of dbSNP build 151 have all 'rsids' (SNP ids)?

dbSNP has got different build (140 to 151). Does the latest version dbSNP build 151 have all snp ids (rsids) that are present in previous build

06 July 2018 4,574 0 View

Is it possible to get odds ratio of a snp from clinvar database?

Some database like ebi gwas provides odds ratio for snps. I wanted to know whether clinvar database provides the same?. I have whole data of clinvar in excel but I can not find odds ratio in it.

05 June 2018 9,591 2 View

What are the best tools for Data Mining Techniques for Harvesting Data from the Internet?

I want to extract the data from the internet with specific medical terms. Which are the available tools with best accuracy or output?

01 February 2018 9,754 7 View

Is combined approach from different research paper a novel methodology?

Consider, 4 research paper with different methodologies to prioritize genes. Combining these methods to single pipeline, can we call it as a "novel method" as together they can not be found in any...

01 February 2018 9,978 2 View

What is the present status of Next Generation sequencing in Clinical Diagnosis?

What is the present status of Next Generation sequencing in Clinical Diagnosis?. Is it well defined to make a conclusion for diagnosis?.

10 November 2017 8,687 3 View

Organs and part of organ classification?

Hi, I think there are about 80 organs in the human body. When I searched for list of all organs different sites are showing different list. Are there any books or publication that clearly mentions...

10 November 2017 950 5 View

Identifying transcript expression from Exome Sequencing?

RNA-Seq is well known for calculating transcript expression. But, researches also do variant calling on RNA-seq data. In such case all mutations can't be identified due to alternative splicing....

07 August 2017 8,057 8 View

RNA-Seq analysis cummerbund package in R?

I performed RNA-Seq data analysis using protocol given in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334321/. When I am using cummerbund package, it shows output like this: CuffSet instance...

07 August 2017 7,088 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to Interpret Partial Eta Squared Numbers For a Repeated Measures Anova?

I have obtained Partial Eta Squared Numbers through Two-Way Mixed Anova Analysis. My results for different constructs are: .105, .135, .038, .068, .061 respectively. I wanted to know, whether...

24 February 2021 7,122 1 View

Can we proceed to perform Two-way Mixed-ANOVA despite normality violations?

While testing assumptions, Shapiro-Wilk Test failed. However, I proceeded with the analysis and the interaction effect was significant. During our course- lectures, it was mentioned that Anova...

24 February 2021 228 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Merga Hailemariam

In our tissue culture experiments we run two different controls (C1 and C2). C1 consists ... There are multiple groups (multiple drugs) to compare to the C2. ... Our qPCR data are normalized ratios as calculated by the LightCycler 480 software.

Abdulmuhsin S. Shihab

There is No need for normalization since all your data have the same unit of measurement. Normalization is conducted in factor analysis as the parameters have different Units.