What is the possibility of replicates being different from other replicates of same sample?

I have analyzed the dataset of GSE38132 from gene expression omnibus. The data is from cell line breast cancer ZR-75-1 which comprises of 9 conditions with 4 replicates for each condition making...

01 February 2020 8,505 4 View

How to perform normalization and calculate when there are multiple groups ?

I have 4 sample [control, pre-treatment, post-treatment, metastais] with 4 replicates which make total 4x4 = 16 samples I want to perform comparison of control with all other groups. For the said...

01 February 2020 5,120 2 View

How does batch correction of gene expression data affect accuracy?

I have two batches of samples which were collected during two time period. If I perform batch correction to remove batch effect will it affect the downstream analysis of gene expression studies?

11 December 2019 1,272 3 View

Does latest version of dbSNP build 151 have all 'rsids' (SNP ids)?

dbSNP has got different build (140 to 151). Does the latest version dbSNP build 151 have all snp ids (rsids) that are present in previous build

06 July 2018 4,574 0 View

What are the best tools for Data Mining Techniques for Harvesting Data from the Internet?

I want to extract the data from the internet with specific medical terms. Which are the available tools with best accuracy or output?

01 February 2018 9,754 7 View

Is combined approach from different research paper a novel methodology?

Consider, 4 research paper with different methodologies to prioritize genes. Combining these methods to single pipeline, can we call it as a "novel method" as together they can not be found in any...

01 February 2018 9,978 2 View

What is the present status of Next Generation sequencing in Clinical Diagnosis?

What is the present status of Next Generation sequencing in Clinical Diagnosis?. Is it well defined to make a conclusion for diagnosis?.

10 November 2017 8,687 3 View

Organs and part of organ classification?

Hi, I think there are about 80 organs in the human body. When I searched for list of all organs different sites are showing different list. Are there any books or publication that clearly mentions...

10 November 2017 950 5 View

Identifying transcript expression from Exome Sequencing?

RNA-Seq is well known for calculating transcript expression. But, researches also do variant calling on RNA-seq data. In such case all mutations can't be identified due to alternative splicing....

07 August 2017 8,057 8 View

RNA-Seq analysis cummerbund package in R?

I performed RNA-Seq data analysis using protocol given in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334321/. When I am using cummerbund package, it shows output like this: CuffSet instance...

07 August 2017 7,088 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

Best excel chart to compare algorithms' running time?

Hi I want to compare the running time of robotic path planning algorithms. The grid size is 8X8. I have 100 scenarios of start and goal positions.. Each scenario is run in three obstacles ratio...

22 February 2021 621 1 View

Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

19 February 2021 8,351 2 View

Convert unit from OR per SD to 1mm for meta analysis?

I would like to ask if someone could help me to convert unit from OR per SD to 1mm for meta analysis. for example The age-adjusted OR per SD cIMT for MMSE

16 February 2021 6,476 2 View

How to open an old ".MTS" file containing spectroscopic data?

Hi supreme hive-mind of the internet, we have some old *.MTS files that are supposed to contain spectroscopic data, probably some coordinates and a long list of single line spectra. Basically,...

16 February 2021 9,385 2 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

How to check if the PMF profile obtained from ABF simulation is correct?

I am doing ABF simulations to calculate ligand binding enegy. The PMF profile that I get using single long ABF simulation is attached as pmf_total.png. When I do this ABF calculation with 10...

10 February 2021 4,170 2 View

What is the Greedy windowed algorithm description?

Hi Dear Ph. D., I have carefully read your paper on anomaly detection in smart meters. Unfortunately, the research does not decribe the greedy windowed algorithm, neither its complexity, nor the...

10 February 2021 2,462 3 View