I want to clone in a gene which is above the packaging limit of the AAV..Is it possible to remove the human beta-globin intron sequence from the pAAV-MCS and replace it with the gene of interest(GOI)? Will it affect the packaging and expression of the GOI from the AAV ?
Bit of a late answer but yes you can take it out if you wish... http://parts.igem.org/Part:BBa_K404107
Dear Stephen.. Thanks for your answer.. I already made a construct without the beta globin and it seems to work too !!
Hi Shambhabi I am wondering whether you saw a decreased expression of GOI when you took away the beta globin intron. Thx
Hello Yuchen,
I had prepared AAV GFP without beta globin intron and I didnot see any difference in terms of GFP expression by microscopic analysis. However, I never checked level of GFP at the protein level after removing beta globin intron.
Best wishes,
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