How to test genetic stability of bacteria?

Hi! I'm looking for a paper or a protocol of a validated method for testing of genetic stability of bacteria. Does anybody know sth like that? We were looking for the papers on this subject but we...

12 January 2021 490 8 View

Is there any easy way to check if given miRNA hybridize to RNA (extracellularly)?

Suppose we have given miRNA (that according to in silico analysis should bind to a specific sequence in RNA) that is labeled with a fluorescent dye (or is labeled otherwise) and we have isolated...

12 November 2020 5,188 1 View

Is anyone interested to join me to the Erasmus project?

I'm Erasmus coordinator from Poland. I'm open to cooperate with another coordinator within Erasmus project. If you are looking for the team, please write to me. Joanna

18 July 2020 7,910 2 View

Is anyone interested in collaborating on an article in the field of effective time management?

I'm looking for someone that is interesting collaboration in effective time management area.

15 July 2020 9,068 5 View

Has anyone done research what are the determinants of implementing industry 4.0 solutions from the point of view of PESTEL factors?

I am looking for research results, scientific articles, tips which factors affect on Industry 4.0 implementation and spread of using in practice. Maybe simple PESTEL factors could be useful -...

17 June 2020 7,494 1 View

What statistical analysis would you recommend to use for my survey data?

Hi everyone, I am currently doing my dissertation for MSc in Strategic Management. My topic focuses on the impact of social media on customer service: case study on Twitter accounts. I have...

24 May 2020 5,275 5 View

What scales could be used to measure wellbeing during the coronavirus pandemic?

I'm looking to find some outcome measures that could address how people feel they are able to cope psychologically during the pandemic and wondered if anyone had any thoughts? Maybe something...

30 April 2020 8,316 3 View

Do I need to address temporal autocorrelation when using boosted regression trees?

I am using boosted regression trees (BRT) to investigate the environmental influences of shark presence at a single location. I have a year-long dataset of acoustic tag detections and...

28 April 2020 914 4 View

Could you help with a survey that concentrates on contact lens behaviours and opinions during COVID-19 pandemy?

Please see the attached invitation to the survey.

25 April 2020 2,635 3 View

What is the possible reason why MM calculation of a protein in Spartan suddenly stopped computing?

I am currently running MM (MMFF94) of a protein-ligand complex for geometry optimization using Spartan '18. The calculation went smoothly until the 19919th cycle when it suddenly stopped computing...

19 March 2020 3,628 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View