How percentage NaCl, sucrose & acid most of the bacteria inhibit or kill ?

Sodium chloride inhibit or kill most of the bacteria at higher concentration without high concentration tolerable bacteria.Similarly lower PH inhibit or kill most of the bacteria without acidofile...

01 February 2017 6,599 3 View

How long time nitrogen activity present in soil ?

If any urea apply on soil it may be lost nitrogen activity within three days.Biofertilizer applied field increase soil nitrogen through nitrate & ammonium producing.I want to know how days or...

31 December 2016 7,957 3 View

What are the easy way to isolate & identification of Green sulfur bacteria ?

I want to be isolate nitrogen fixing green sulfur bacteria.which technique apply i quickly identify green sulfer bacteria.

11 December 2016 7,263 6 View

Why bacteria fix nitrogen from atmosphere ?

We know that nitrogen fixing bacteria fix nitogen from atmosphere.I want to know why bacteria fix nitrogen. Bacteria need nitrogen for their growth for this reason nor their metabolism.Is it...

11 December 2016 4,512 5 View

Why trichoderma spore produce & what are the crucial factor that affect the high spore production of trichoderma?

09 October 2016 6,418 4 View

How I can count of bacteria & spore of Trichoderma through spectrophotometer ?

I know exact count possible by CFU/ml through spread plate count method & Haemocytometer count. But I want to know count bacteria & spore through spectrophotometer.But how is it possible...

07 August 2016 8,055 2 View

What are the easy way to visible bacteria count through haemocytometer ?

Instantly cell count is most important to microbiology.But very deficult to distinguish solid particle & bacteria.I want to know have any dye to use in this count through haemocytometer.Please...

07 August 2016 9,852 0 View

Which Rhizobia spp. create nodule of Country bean & groundnut plant ?

Recently i work on rhizobia spp. isolation & identification.Recently i get a isolate which can create nodule groundnut & country bean (BARI Seem 5 in Bangladesh).Which bacteria may be in...

07 August 2016 2,616 2 View

Which rhizobia bacteria create nodule at chickpea & groundnut/peanut separately ?

06 July 2016 9,614 2 View

How we are create nodule artificially on leguminous plant in vitro tissue culture method ?

Nodule creation by in vitro tissue culture method.

04 May 2016 1,471 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View