How percentage NaCl, sucrose & acid most of the bacteria inhibit or kill ?

Sodium chloride inhibit or kill most of the bacteria at higher concentration without high concentration tolerable bacteria.Similarly lower PH inhibit or kill most of the bacteria without acidofile...

01 February 2017 6,599 3 View

How long time nitrogen activity present in soil ?

If any urea apply on soil it may be lost nitrogen activity within three days.Biofertilizer applied field increase soil nitrogen through nitrate & ammonium producing.I want to know how days or...

31 December 2016 7,957 3 View

What are the easy way to isolate & identification of Green sulfur bacteria ?

I want to be isolate nitrogen fixing green sulfur bacteria.which technique apply i quickly identify green sulfer bacteria.

11 December 2016 7,263 6 View

Why bacteria fix nitrogen from atmosphere ?

We know that nitrogen fixing bacteria fix nitogen from atmosphere.I want to know why bacteria fix nitrogen. Bacteria need nitrogen for their growth for this reason nor their metabolism.Is it...

11 December 2016 4,512 5 View

Why trichoderma spore produce & what are the crucial factor that affect the high spore production of trichoderma?

09 October 2016 6,418 4 View

How I can count of bacteria & spore of Trichoderma through spectrophotometer ?

I know exact count possible by CFU/ml through spread plate count method & Haemocytometer count. But I want to know count bacteria & spore through spectrophotometer.But how is it possible...

07 August 2016 8,055 2 View

What are the easy way to visible bacteria count through haemocytometer ?

Instantly cell count is most important to microbiology.But very deficult to distinguish solid particle & bacteria.I want to know have any dye to use in this count through haemocytometer.Please...

07 August 2016 9,852 0 View

Is it possible to produce pure culture of Trichoderma viride, If possible so what are the way?

Recently I work on Trichoderma viride, when it culture on PDA plate, that's time no vissible bacteria.But when culture trichoderma production browth media, there present bacteria.This bacteria is...

07 August 2016 3,523 2 View

Which rhizobia bacteria create nodule at chickpea & groundnut/peanut separately ?

06 July 2016 9,614 2 View

How we are create nodule artificially on leguminous plant in vitro tissue culture method ?

Nodule creation by in vitro tissue culture method.

04 May 2016 1,471 4 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View