Recently i work on rhizobia spp. isolation & identification.Recently i get a isolate which can create nodule groundnut & country bean (BARI Seem 5 in Bangladesh).Which bacteria may be in this isolate i want to know.
Please have a look at these useful links for groundnut
http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000629
https://www.researchgate.net/publication/234085945_Bradyrhizobium_arachidis_sp_nov_isolated_from_effective_nodules_of_Arachis_hypogaea_grown_in_China
https://www.researchgate.net/publication/280393243_Bradyrhizobium_subterraneum_sp_nov_a_symbiotic_nitrogen-fixing_bacterium_from_root_nodules_of_groundnuts_in_Namibia
https://www.researchgate.net/publication/268232895_Rhizobium_pakistanensis_sp_nov_isolated_from_groundnut_Arachis_hypogaea_nodules_grown_in_rainfed_Pothwar_Pakistan
https://www.researchgate.net/publication/12070338_Genetic_diversity_of_Bradyrhizobium_strains_isolated_from_Arachis_hypogaea
https://www.researchgate.net/publication/222497998_Nodulation_in_peanut_Arachis_hypogaea_L_roots_in_the_presence_of_native_and_inoculated_rhizobia_strains
http://link.springer.com/article/10.1007/s11104-005-5314-5
Article Bradyrhizobium arachidis sp. nov., isolated from effective n...
Article Bradyrhizobium subterraneum sp. nov., a symbiotic nitrogen-f...
Article Rhizobium pakistanensis sp. nov., isolated from groundnut (A...
Article Genetic diversity of Bradyrhizobium strains isolated from Ar...
Article Nodulation in peanut (Arachis hypogaea L.) roots in the pres...
Thank you very much Mr. Arvind Singh
Sodium chloride inhibit or kill most of the bacteria at higher concentration without high concentration tolerable bacteria.Similarly lower PH inhibit or kill most of the bacteria without acidofile...
01 February 2017 6,760 3 View
If any urea apply on soil it may be lost nitrogen activity within three days.Biofertilizer applied field increase soil nitrogen through nitrate & ammonium producing.I want to know how days or...
31 December 2016 8,064 3 View
I want to be isolate nitrogen fixing green sulfur bacteria.which technique apply i quickly identify green sulfer bacteria.
11 December 2016 7,368 6 View
We know that nitrogen fixing bacteria fix nitogen from atmosphere.I want to know why bacteria fix nitrogen. Bacteria need nitrogen for their growth for this reason nor their metabolism.Is it...
11 December 2016 4,638 5 View
Spore production of trichoderma is important for industrial scale.What are the way to improve high spore production.Please suggest me?
09 October 2016 6,513 4 View
I know exact count possible by CFU/ml through spread plate count method & Haemocytometer count. But I want to know count bacteria & spore through spectrophotometer.But how is it possible...
07 August 2016 8,245 2 View
Instantly cell count is most important to microbiology.But very deficult to distinguish solid particle & bacteria.I want to know have any dye to use in this count through haemocytometer.Please...
07 August 2016 9,965 0 View
Recently I work on Trichoderma viride, when it culture on PDA plate, that's time no vissible bacteria.But when culture trichoderma production browth media, there present bacteria.This bacteria is...
07 August 2016 3,628 2 View
Recently, I work rhizobia bacteria which can create nodule at chikpea & groundnut root.But can't sequencing of this isolate.But some biochemical test perform of this isolate.I want to know...
06 July 2016 9,764 2 View
Nodule creation by in vitro tissue culture method.
04 May 2016 1,627 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View