How percentage NaCl, sucrose & acid most of the bacteria inhibit or kill ?

Sodium chloride inhibit or kill most of the bacteria at higher concentration without high concentration tolerable bacteria.Similarly lower PH inhibit or kill most of the bacteria without acidofile...

01 February 2017 6,599 3 View

What are the easy way to isolate & identification of Green sulfur bacteria ?

I want to be isolate nitrogen fixing green sulfur bacteria.which technique apply i quickly identify green sulfer bacteria.

11 December 2016 7,263 6 View

Why bacteria fix nitrogen from atmosphere ?

We know that nitrogen fixing bacteria fix nitogen from atmosphere.I want to know why bacteria fix nitrogen. Bacteria need nitrogen for their growth for this reason nor their metabolism.Is it...

11 December 2016 4,512 5 View

Why trichoderma spore produce & what are the crucial factor that affect the high spore production of trichoderma?

09 October 2016 6,418 4 View

How I can count of bacteria & spore of Trichoderma through spectrophotometer ?

I know exact count possible by CFU/ml through spread plate count method & Haemocytometer count. But I want to know count bacteria & spore through spectrophotometer.But how is it possible...

07 August 2016 8,055 2 View

What are the easy way to visible bacteria count through haemocytometer ?

Instantly cell count is most important to microbiology.But very deficult to distinguish solid particle & bacteria.I want to know have any dye to use in this count through haemocytometer.Please...

07 August 2016 9,852 0 View

Which Rhizobia spp. create nodule of Country bean & groundnut plant ?

Recently i work on rhizobia spp. isolation & identification.Recently i get a isolate which can create nodule groundnut & country bean (BARI Seem 5 in Bangladesh).Which bacteria may be in...

07 August 2016 2,616 2 View

Is it possible to produce pure culture of Trichoderma viride, If possible so what are the way?

Recently I work on Trichoderma viride, when it culture on PDA plate, that's time no vissible bacteria.But when culture trichoderma production browth media, there present bacteria.This bacteria is...

07 August 2016 3,523 2 View

Which rhizobia bacteria create nodule at chickpea & groundnut/peanut separately ?

06 July 2016 9,614 2 View

How we are create nodule artificially on leguminous plant in vitro tissue culture method ?

Nodule creation by in vitro tissue culture method.

04 May 2016 1,471 4 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View