Instantly cell count is most important to microbiology.But very deficult to distinguish solid particle & bacteria.I want to know have any dye to use in this count through haemocytometer.Please anyone advise me?
Sodium chloride inhibit or kill most of the bacteria at higher concentration without high concentration tolerable bacteria.Similarly lower PH inhibit or kill most of the bacteria without acidofile...
01 February 2017 6,760 3 View
If any urea apply on soil it may be lost nitrogen activity within three days.Biofertilizer applied field increase soil nitrogen through nitrate & ammonium producing.I want to know how days or...
31 December 2016 8,064 3 View
I want to be isolate nitrogen fixing green sulfur bacteria.which technique apply i quickly identify green sulfer bacteria.
11 December 2016 7,368 6 View
We know that nitrogen fixing bacteria fix nitogen from atmosphere.I want to know why bacteria fix nitrogen. Bacteria need nitrogen for their growth for this reason nor their metabolism.Is it...
11 December 2016 4,638 5 View
Spore production of trichoderma is important for industrial scale.What are the way to improve high spore production.Please suggest me?
09 October 2016 6,513 4 View
I know exact count possible by CFU/ml through spread plate count method & Haemocytometer count. But I want to know count bacteria & spore through spectrophotometer.But how is it possible...
07 August 2016 8,245 2 View
Recently i work on rhizobia spp. isolation & identification.Recently i get a isolate which can create nodule groundnut & country bean (BARI Seem 5 in Bangladesh).Which bacteria may be in...
07 August 2016 2,747 2 View
Recently I work on Trichoderma viride, when it culture on PDA plate, that's time no vissible bacteria.But when culture trichoderma production browth media, there present bacteria.This bacteria is...
07 August 2016 3,628 2 View
Recently, I work rhizobia bacteria which can create nodule at chikpea & groundnut root.But can't sequencing of this isolate.But some biochemical test perform of this isolate.I want to know...
06 July 2016 9,764 2 View
Nodule creation by in vitro tissue culture method.
04 May 2016 1,627 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View