Is it possible to perform proteomic analysis of paraformaldehyde-fixed samples on slides?

12 May 2023 1 6K Report

How can these samples be extracted from the slide for further analysis? Any references? Protocol?

You can digest the FFPE samples with trypsin and you will often get some peptides. However, many of the peptides will be corrupted by the formalin fixation reaction with some peptides being crosslinked to others. First, you have to de-paraffin the samples. There was a high-pressure process developed by Fowler, but we could never get this to work in our hands.Article Pressure-Assisted Protein Extraction: A Novel Method for Rec...

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