The sample used for Widal test is SERUM.
When you want to collect sample for Widal, you need to seperately collect blood and separate serum out of it and perform Widal which detects the Antibodies produced against Typhoidal Salmonella Antigen.
Of course you can use the blood culture sample for PCR to detect Typhoidal Salmonella (Ag), if you need to rule out typhoid or paratyphoid.
Maybe. Why would your "blood culture" produce Salmonella antibodies? If it does, you've got yourself one interesting little blood culture :)
I would try different dilutions, to make sure you have something that ends up within the dynamic range of the assay. Also concentrations, e.g. with a 100k concentration filter.
I'm guessing the best way to do this would be some kind of ELISA-like design.
What is your goal?
The question is little doubt is it from blood culture or for blood culture?
If it is for blood culture......
The answer is During the sample collection you can keep 2 ml blood separately for serum which will be tested for Widal test for typhoid.
If it from blood culture...
It is little difficult. The blood is already mixed with 10 times culture medium where dilution factor will affect and some time it will missing if lower titre of antibodies is present. in the whole blood
If I am not wrong Widal test is based upon antigen antibody interaction. Then ideally we should use serum not whole blood. You need to possibly explore your question as it is somewhat difficult to understand what exactly you want to know.
I know Widal requires serum not whole blood. If he can use from blood culture, he can purify 1/10 diluted plasma from blood culture and adding calcium chloride to plasma he can make serum and use as 1/10 diluted. serum
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