Is it possible to get SPR in the thickness of 150-200 nm? From the uv-vis spectroscopy graph I can not understand,immobilization is correct or not?

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Geeta Sharma

It is very difficult to get SPR with thick and bigger structures and if you want to check this do the SPR measurement with prism and white light lamp . You can refer raether book for this . You will get better idea.

Mohamed Elderdfi

I would go with @Jon Ashley, to calculate the thickness using the SPR Navi LayerSolver software or XPS