hey you can not get peak of virus. In chromatography we can check different compounds not a species. so we can not able to check virus. yes you can check the presence of virus by checking any signature compound present in that particular virus.

Thanks Nitesh but if the virus contains any glycoprotein or if any native structure of protein that can show peak?

You didn't tell about which chromatography you are using for your work. For protein separation and identification different chromatography can use according to nature of protein.

This is mainly a column chromatograhhy system where different agarose or cellulose base matrix can use for separation mainly in size exclusion and affinity.

Hi - depending on the concentration and purity of your virus preparation you might find it useful to do capillary electrophoresis. If you're interested you can check for instance the work of the group of Ernst Kenndler, University of Vienna and Dieter Blaas, Medical Univrsity of Vienna. They have analyzed intact human Rhinovirus with CE. Virus peak identification was possible through (i) heat denaturation and (ii) affinity experiments. Subsequently, the electrophoretic mobility was sufficient to check for the peak of intact virions. Maybe you can also port some concepts of their CE work to LC.

Thanks Victor. Could you please send me the link of the work of the group of Ernst Kenndler, University of Vienna and Dieter Blaas, Medical Univrsity of Vienna.

Could you please also tell me about affinity experiment that how could it be possible to detect the peak of a virus as UV spectra is not able to do that.