Is it possible to do multiple sterotaxic injections into a mouse brain?

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Linear vs Supercoiled vs Nicked DNA on agarose?

I am pretty new to molecular biology techniques and just when I think I have it all figured out, something stumps me. This is how this gel (1.5% agarose) is loaded: 1. GeneRuler ready to use cat#...

06 October 2020 7,523 4 View

Has anyone reported on carbon storage potential in common trees and shrubs in prairie forests?

Has anyone reported on carbon storage potential in common trees and shrubs in prairie forests? I am looking for carbon storage potential ie carbon biomass for a number of native trees and shrubs...

08 July 2020 5,236 2 View

Issues with Image J Thresholding?

I am wondering if anyone has had issues with their image J thresholding? I am trying to perform colour deconvolution, but when I convert the image to binary, I am unable adjust the threshold to...

01 April 2020 3,812 3 View

Where can I get survey data to compare the US to subSaharan Africa, China on the Impact of Climate Change?

Preparing a research paper. Other countries/geographies could be substituted

12 March 2020 195 4 View

Quantifying ADC values from a ROI?

I currently have both DWI and ADC sequences however I want to calculate the mean ADC value for a defined ROI (ischemic stroke lesion). I currently use ITK-SNAP, Image J and Horos for viewing...

03 March 2020 9,749 4 View

Which software is good for analysis of band size in gel image? How can we analyze the curve band image?

Currently, i am thinking about GelAnalyzer software. Please help me with the analysis and band scoring. I have used 100 bp ladder.

17 February 2020 7,762 5 View

How to apply fuzzy K-means with spatial constraints in R?

Hello. I am looking for a solution in R to apply spatially-constrained clustering using fuzzy K-means (fuzzy c-means). I came across the spatialClust package, and was wondering whether anyone may...

30 October 2019 4,701 2 View

How to apply fuzzy K-means with spatial constraints in R?

Hello. I am looking for a solution in R to apply spatially-constrained clustering using fuzzy K-means (fuzzy c-means). I came across the spatialClust package, and was wondering whether anyone may...

09 October 2019 8,487 3 View

How do I align a sequence set to one reference sequence?

I am currently trying to find homologues of a protein I am working with, but BLAST has been giving me nothing useable. I have now found a dataset of 1500 protein sequences of potential candidates...

26 June 2019 5,596 2 View

I need an analytical balance that weighs organic samples at 0.0001 g for stable isotope analysis. What models are reliable and cost-effective?

For weighing stable isotope samples

18 October 2018 687 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View