Levi Adams

6 Questions 6 Answers 0 Followers

Questions related from Levi Adams

What percent of Glioblastoma studies use the U87 cell line?

I'm studying glioblastoma and I can't help but notice that a large majority of the basic research papers use this one cell line. Is there a resource anyone knows about that would allow to to see...

01 February 2018 1,613 3 View

How can I recover 3D cultures from Matrigel for a phospho-Western Blot?

I am studying vasculogenic potential of cancer stem cells, and so I culture stem cell spheres on Matrigel. After several days, they form 3D tube structures into the gel. Inhibition of my protein...

29 November 2017 8,987 8 View

How do I minimize oxygen flux when performing routine cell culture tasks on hypoxic cultures?

I am beginning to perform several experiments using cancer cells in a hypoxic environment. Our core facility has a hypoxic incubator that uses nitrogen to displace oxygen down to a minimum of 1%...

19 October 2017 6,487 3 View

Anyone have problems with PCR of LoxP inserts?

I've been trying to subclone a LoxP-DsRed-LoxP insert, and I'm having a lot of difficulty with the PCR. I played with the annealing temp and tried adding DMSO and High GC enhancer, but no...

10 June 2016 8,384 7 View

Any advice on cloning a 2A sequence between 2 genes without restriction enzymes?

I'm trying to clone several genes into one plasmid, and was looking at using a 2A self cleaving sequence because its short. So: start-Gene A -- 2A-- Gene B - stop As far as the actual cloning -...

11 November 2015 6,751 2 View

Is it possible to do multiple sterotaxic injections into a mouse brain?

I am working on an endogenous brain tumor model and will induce tumor formation by stereotaxic injection. Part of the treatment regimen will also be delivered via stereotaxic injection into the...

20 July 2015 4,703 3 View