Is it possible to conserve plant leaves for DNA extraction in a freezer at 40 C for 1 week rather than freezer 80 C after fall into liquid nitrogen?

More Laiadi Ziane's questions See All

Entropy ans phase transition ?

There is a concrete relationship between entropy and phase transition in semiconductor alloys ?

13 March 2023 3,078 3 View

Respectful colleagues, How can I have an equivalency in exercise physiologist by my Phd in methodologies and theories of sport training diploma?

Our structure diplima in our county have the sport for health in under secondary specialty (much more for handisport), but it had not a public nor liberal status, that's why I would make a...

10 December 2022 7,295 4 View

I Want to add some axis (questions, items) to my organizational commitment survey, to know if employee commitment is result of managers competency?

Dear researchers, I Want to add some axis (questions, items) to my organizational commitment survey, to know if employee commitment is the result of managers competency? First topics of my design...

23 July 2018 1,751 10 View

What is the best program used to simulate and study multilayer solar cells ??

I work on materials used for photovoltaic applications. I am looking for a physical program to use for simulate and determine the properties of a solar cell.

13 June 2018 9,476 28 View

How to calculate the transmission from complex dielectric function (real and imaginary part) ?

Hello, We used the wien2k code for predicting the optical properties of cristallin material. We can obtained the complex dielectric function (real and imaginary part) and other quantities but not...

22 June 2017 4,224 2 View

How to grind plastic ?

hello everyone; Could you offered me a technical in order to grind plastic and make it in powder form. Thnx

01 April 2016 5,943 17 View

How we can use epsilon constraint method for the multi-objective optimization?

I want to know the uses of epsilon constraint method for finding the optimal solution of multi-objective functions.

03 September 2015 1,572 5 View

Is normal distribution is required in exploratory and confirmatory factor analysis in survey?

Dear researchers I am using spss is normal distribution is required in exploratory and confirmatory factor analysis in survey? I have at least 4 items for each axies and 7 at maximum, I ask when...

01 January 1970 8,165 10 View

What is the alternative of "one sample t test" of variable is not normal distributed

Hi Dear researchers I Ask you to help me to solve a problem found in my statistic, the information will be shown bellow: My thesis titled: "studying the impact of manager's competency on...

01 January 1970 4,788 11 View

How can I interprete results of kruskal wallis?

Hi I wondering about how to interpret results in model view (after double cliking) on results, when paireswise chart with orange color of pairs, and the table after with some values with orange...

01 January 1970 4,575 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Laiadi Ziane

May be

Nikolay Klyashtornyy

Even -20 C would work well.

Nicola Cook

I'm sure that would work well, I have previously flash-frozen plant tissue and then stored at -20 degrees as Nikolay suggests and used in DNA extraction with success.

Nian Wang

It will work. I extracted plant DNA from pressed specimens for over a couple of months and it worked well.