Nicola Cook

3 Questions 10 Answers 0 Followers

Questions related from Nicola Cook

Why would an RNA extract be viscous?

A student in my department has recently submitted some RNA samples to a sequencing centre for NGS. He was advised that there were problems with the library prep due to vicosity of the RNA samples....

06 February 2018 6,437 7 View

Will RNAlater work with small insect with very hard exoskeleton?

I will be setting up an experiment to assay gene expression in the brains (effectively heads) of my wasps (body length of wasp: head to abdomen c. 2mm). Due to the large volume of tissue required...

12 May 2015 1,634 15 View

Has anyone ever observed two pellets after a phenol/chloroform DNA extraction?

I am using a standard phenol chloroform extraction protocol to extract DNA from very small amounts of insect tissue. I have included extra steps (KAc treatment prior to phenol step) to remove...

14 November 2013 6,975 7 View