I didn't want my samples to be too dilute so I extracted my samples by adding 5ul RIPA per mg tissue. Now I'm concerned that I haven't added enough RIPA buffer as it seems like everyone uses at least 10ul RIPA per mg tissue. I've run the blots and they look perfectly fine, but I'm paranoid that having too little RIPA buffer could affect my results.
5ul/mg seems too little - downside is you missed to solubilize everything but whatever you did you are satisfied with the result then you dont have to worry.
If the experiment is reproducible, add 10ul RIPA buffer this time repeat and compare.
You should also consider the protein concentration.The minimum recommended protein concentration is 0.1 mg/ml and optimal concentration is considered as 1-5 mg/ml.
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