Shi Li

2 Questions 6 Answers 0 Followers

Questions related from Shi Li

Is it possible to add too little RIPA buffer to tissues for western blot tissue extraction? If so, what are the consequences?

I didn't want my samples to be too dilute so I extracted my samples by adding 5ul RIPA per mg tissue. Now I'm concerned that I haven't added enough RIPA buffer as it seems like everyone uses at...

04 April 2019 5,490 4 View

Does anyone have a protocol for tissue preparation and staining of endothelial cells in rat brain sections?

I'm having some trouble trying to stain for endothelial cell in rat brain tissues. My positive control works for CD31, but my samples are not coming up. I'm wondering if it is my tissue...

07 July 2017 9,908 4 View