Is it possible that VeriSeq (Illumina) kit is not intended for DNA, extracted from blood?

02 February 2018 1 2K Report

We sequenced embryos for aneuploidy screening, and also for control took one genomic DNA sample, previously known with normal karyotype. All the samples were amplified with SurePlex system. After NGS on MiSeq with VeriSeq kit, this sample with normal karyotype had a trisomy of chr19 (the profiles of embryos looked as they should look). Is it possible, that there might be any amplification errors due to wide regions of euchromatin in chr19, and VeriSeq with Miseq is not suitable for testing usual DNA from leukocytes?

Abhijeet Singh

Hi,

It is really hard to comment on this problem, without having a closer look on the data and troubleshooting the protocol you have used. Yet, have you considered the possibility of the contamination, since there is a specific technical note available from Illumina for the sample preparation and troubleshooting. Please have a look on it, hope this will help.

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Science method

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