I'm working with Macherey-Nagel kit
Don't know if you can get larger fragments from FFPE-samples ... Do you use punched samples or section slides ....
Promega ReliaPrep are quite good. Work better than many competitors.
https://www.promega.co.uk/products/dna-purification-quantitation/genomic-dna-purification/reliaprep-ffpe-gdna-miniprep-system/
However, you cannot expect very high quality DNA from FFPE, especially if your samples are old.
Dear Baiba, you can try to use QIAamp DNA FFPE Tissue Kit (qiagen) or promega for DNA isolation. I agree with Maxim Freidin, with time nucleic acids especially RNA can degrade in FFPE tissue.
The following paper can also help your studies.
Virchows Arch. 2012 Feb;460(2):131-40. doi: 10.1007/s00428-011-1184-9.
Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses.
Best regards.
We sequenced embryos for aneuploidy screening, and also for control took one genomic DNA sample, previously known with normal karyotype. All the samples were amplified with SurePlex system. After...
01 February 2018 1,956 1 View
There was an issue with my thermal cycler
07 November 2017 9,104 3 View
I've done everything as described in manual, but the PCR still is not working. There is amplification only in specific regions, but all the positive controls remain empty.
03 September 2017 5,418 2 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View