Do Anti-APC microbeads cross react with PE or Pe-Cy7 labelled antibodies?

I have a suspension of cells labelled with FITC, PE, Pe-Cy7 and APC conjugated antibodies. I want to isolate APC labelled cells with a kit containing Anti APC microbeads. APC and PE have a...

03 April 2013 2,655 3 View

Is possible to detect antigen specific CD4 T cells with tetramers following primary activation?

I would like to stain with MHC II tetramers specific T cells following primary activation, could I detect this cells? Does anyone have some advice for my protocol?

02 March 2013 291 2 View

How could I study the expansion of a population of antigen-specific B cells?

Is it possible to use fluorescent antigen to detect antigen-specific B cells?

02 March 2013 1,073 1 View

How many HT-29 cells could I seed on a 6-well plate?

I would like to study the expression of a protein following stimulation with bacterial components.

31 December 2012 1,367 3 View

Which are the best in vitro assays to check probiotic strain activity?

I need to check probiotic activity

10 November 2012 9,069 11 View

How could I track the pool of antigen specific CD4 T cells following immunization?

Can you suggest methods alternative to T cell adoptive transfer?

08 September 2012 4,128 25 View

Do lymphocytes express CD11c?

I need to identify dendritic cells in a lymph nodes. If both lymphocytes and DC express CD11c is it possible to discriminate it from lymphocytes by physycs parameter?

08 September 2012 5,425 8 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

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03 March 2021 5,360 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View