Is it possible that T cells down regulated CD62L following a 2 hours incubation at 37 C?

I've incubated cells from mice lymph nodes for 2 hours at 37 C for MHC II tetramer staining, in my samples I could not detect CD62L positive cells also in naive mice.

05 June 2013 3,200 2 View

Do Anti-APC microbeads cross react with PE or Pe-Cy7 labelled antibodies?

I have a suspension of cells labelled with FITC, PE, Pe-Cy7 and APC conjugated antibodies. I want to isolate APC labelled cells with a kit containing Anti APC microbeads. APC and PE have a...

03 April 2013 2,655 3 View

How could I study the expansion of a population of antigen-specific B cells?

Is it possible to use fluorescent antigen to detect antigen-specific B cells?

02 March 2013 1,073 1 View

How many HT-29 cells could I seed on a 6-well plate?

I would like to study the expression of a protein following stimulation with bacterial components.

31 December 2012 1,367 3 View

Which are the best in vitro assays to check probiotic strain activity?

I need to check probiotic activity

10 November 2012 9,069 11 View

How could I track the pool of antigen specific CD4 T cells following immunization?

Can you suggest methods alternative to T cell adoptive transfer?

08 September 2012 4,128 25 View

Do lymphocytes express CD11c?

I need to identify dendritic cells in a lymph nodes. If both lymphocytes and DC express CD11c is it possible to discriminate it from lymphocytes by physycs parameter?

08 September 2012 5,425 8 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

No title

03 March 2021 5,360 2 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Is the sinovac vaccine safe and effective for people over the age of 60 ?

Dear respective researchers, I was asked by my institution to be vaccinated this week using the sinovac vaccine. However, from some of the information I got, this vaccine is not recommended for...

01 March 2021 7,781 6 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View