Gennaro Prota

8 Questions 6 Answers 0 Followers

Questions related from Gennaro Prota

Is it possible that T cells down regulated CD62L following a 2 hours incubation at 37 C?

I've incubated cells from mice lymph nodes for 2 hours at 37 C for MHC II tetramer staining, in my samples I could not detect CD62L positive cells also in naive mice.

06 June 2013 3,211 2 View

Do Anti-APC microbeads cross react with PE or Pe-Cy7 labelled antibodies?

I have a suspension of cells labelled with FITC, PE, Pe-Cy7 and APC conjugated antibodies. I want to isolate APC labelled cells with a kit containing Anti APC microbeads. APC and PE have a...

04 April 2013 2,664 3 View

How could I study the expansion of a population of antigen-specific B cells?

Is it possible to use fluorescent antigen to detect antigen-specific B cells?

03 March 2013 1,091 1 View

Is possible to detect antigen specific CD4 T cells with tetramers following primary activation?

I would like to stain with MHC II tetramers specific T cells following primary activation, could I detect this cells? Does anyone have some advice for my protocol?

03 March 2013 301 2 View

How many HT-29 cells could I seed on a 6-well plate?

I would like to study the expression of a protein following stimulation with bacterial components.

01 January 2013 1,379 3 View

Which are the best in vitro assays to check probiotic strain activity?

I need to check probiotic activity

11 November 2012 9,086 11 View

Do lymphocytes express CD11c?

I need to identify dendritic cells in a lymph nodes. If both lymphocytes and DC express CD11c is it possible to discriminate it from lymphocytes by physycs parameter?

09 September 2012 5,436 8 View

How could I track the pool of antigen specific CD4 T cells following immunization?

Can you suggest methods alternative to T cell adoptive transfer?

09 September 2012 4,142 25 View