Is it possible that T cells down regulated CD62L following a 2 hours incubation at 37 C?

I've incubated cells from mice lymph nodes for 2 hours at 37 C for MHC II tetramer staining, in my samples I could not detect CD62L positive cells also in naive mice.

05 June 2013 3,200 2 View

Do Anti-APC microbeads cross react with PE or Pe-Cy7 labelled antibodies?

I have a suspension of cells labelled with FITC, PE, Pe-Cy7 and APC conjugated antibodies. I want to isolate APC labelled cells with a kit containing Anti APC microbeads. APC and PE have a...

03 April 2013 2,655 3 View

Is possible to detect antigen specific CD4 T cells with tetramers following primary activation?

I would like to stain with MHC II tetramers specific T cells following primary activation, could I detect this cells? Does anyone have some advice for my protocol?

02 March 2013 291 2 View

How could I study the expansion of a population of antigen-specific B cells?

Is it possible to use fluorescent antigen to detect antigen-specific B cells?

02 March 2013 1,073 1 View

Which are the best in vitro assays to check probiotic strain activity?

I need to check probiotic activity

10 November 2012 9,069 11 View

How could I track the pool of antigen specific CD4 T cells following immunization?

Can you suggest methods alternative to T cell adoptive transfer?

08 September 2012 4,128 25 View

Do lymphocytes express CD11c?

I need to identify dendritic cells in a lymph nodes. If both lymphocytes and DC express CD11c is it possible to discriminate it from lymphocytes by physycs parameter?

08 September 2012 5,425 8 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View