Is it possible that T cells down regulated CD62L following a 2 hours incubation at 37 C?

I've incubated cells from mice lymph nodes for 2 hours at 37 C for MHC II tetramer staining, in my samples I could not detect CD62L positive cells also in naive mice.

05 June 2013 3,200 2 View

Do Anti-APC microbeads cross react with PE or Pe-Cy7 labelled antibodies?

I have a suspension of cells labelled with FITC, PE, Pe-Cy7 and APC conjugated antibodies. I want to isolate APC labelled cells with a kit containing Anti APC microbeads. APC and PE have a...

03 April 2013 2,655 3 View

Is possible to detect antigen specific CD4 T cells with tetramers following primary activation?

I would like to stain with MHC II tetramers specific T cells following primary activation, could I detect this cells? Does anyone have some advice for my protocol?

02 March 2013 291 2 View

How many HT-29 cells could I seed on a 6-well plate?

I would like to study the expression of a protein following stimulation with bacterial components.

31 December 2012 1,367 3 View

Which are the best in vitro assays to check probiotic strain activity?

I need to check probiotic activity

10 November 2012 9,069 11 View

How could I track the pool of antigen specific CD4 T cells following immunization?

Can you suggest methods alternative to T cell adoptive transfer?

08 September 2012 4,128 25 View

Do lymphocytes express CD11c?

I need to identify dendritic cells in a lymph nodes. If both lymphocytes and DC express CD11c is it possible to discriminate it from lymphocytes by physycs parameter?

08 September 2012 5,425 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View