Is it possible ? - FAQS.TIPS

More Tomasz Hura's questions See All

Research paradigm of systematic review?

Hi, I am doing a systematic literature review for my MPhil. Does anyone know what philosophy/research paradigm is a systematic review?

18 June 2024 9,525 1 View

How to do research on Polish people in the UK prison?

Hi I am a student in the UK, want to do my PhD research in prison in the UK interviewing Polish people in Polish language do you have any idea how I could facilitate this? How to get access to the...

14 February 2024 8,599 5 View

Should I replace SV40 poly(A) signal with my 3'UTR of choice?

I want to test few 5'UTRS and 3'UTRs on their effect on luciferase expression. I will be performing in-vitro transcription to generate mRNA. I am using a luciferase vector with T7 promoter and...

07 December 2023 7,033 1 View

What homogenisation & DNA extraction method/kit should I use to perform metabarcoding of endophytic plant microbiome (roots, leaves, seeds, soil)?

Hi, I plan to conduct detailed research on the endophytic microbiome in one of Apiaceae members. I will collect root, leaf and seed samples to study endophytic microbiome. I will also analyse soil...

05 December 2023 5,065 0 View

Dnase I working conditions ?

I would like to kindly ask for help. I need to prepare great amount of Dnase I solution out of the premade stock (c.a 45 ml of 40U/ml). What is the best composistion of diluting solution? I read...

03 December 2023 1,545 0 View

Does TT cell line (medullary thyroid cancer cell line) express VEGF receptor ?

In ATCC this cell line is also described as CRL-1803.

25 October 2023 7,741 1 View

What is enthalpy of NH3 vaporation (kJ/mol) from its aq. solution?

Hello, I'm looking for how much energy I need to evaporate 1 mole of NH3 from its aq. solution (for example 6%, at STP ) or how can I calculate this energy? To be honest, I'm not sure If this...

31 August 2023 9,930 0 View

How to measure scanned thin-sections?

please help me to find a software to measure distances - areas and angles in scans of thin-sections, ie flat pictures... in my case sectioned were fossil corals... 1 I have resolution and size of...

12 June 2023 9,804 1 View

How to separate sand from pulverised biomass?

I have powdered biomass that I want to analyse for heavy metal content. However, the biomass is contaminated by soil residues, which may contribute to the overall heavy metal content of the...

11 June 2023 4,151 0 View

What is the best culture medium for primary human endothelial cells culture ?

I'm looking for the best choice of ready to use culture media for human endothelial cells isolated from aorta?

25 March 2023 4,746 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View